IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS (MTB) IN SPUTUM OF PATIENTS WITH COUGH ≥ 2 WEEKS USING ZIEHL- NEELSEN STAINING METHOD AT RSUD SITI FATIMAH AZ-ZAHRA PALEMBANG IN 2025

M Nabil; Teni Nopita Sari

doi:10.62567/micjo.v3i1.2330

Authors

M Nabil Universitas Kader Bangsa
Teni Nopita Sari Universitas Kader Bangsa

DOI:

https://doi.org/10.62567/micjo.v3i1.2330

Keywords:

Tuberculosis, Cough ≥ 2 weeks, Sputum, Ziehl-Neelsen, AFB

Abstract

Tuberculosis remains a major public health problem in Indonesia with high morbidity and mortality rates. This study aimed to identify Mycobacterium Tuberculosis (MTB) in sputum samples of patients with productive cough lasting

≥ 2 weeks using the Ziehl-Neelsen staining method at RSUD Siti Fatimah Az- Zahra Palembang. This study uses a descriptive observational method with a cross-sectional approach and involves 30 respondents. The results showed that all respondents experienced cough, with the majority 24 respondents (80.0%) having productive cough ≥ 2 weeks and 6 respondents (20.0%) having prolongrd cough < 2 weeks. Sputum examination revealed 17 respondents (56.7%) were Acid-Fast Bacilli (AFB) positive, while 13 respondents (43.3%) were negative. These findings indicate that a prolonged cough lasting ≥ 2 weeks is associated with AFB-positive results, although tuberculosis may also occur in patients with prolonged cough lasting < 2 weeks. It is recommended that patients with positive results undergo complete DOTS therapy, hospitals improve diagnostic services and health education, communities adopt a healthy lifestyle, and further research be conducted to explore the underlying causes of prolonged cough.

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References

Alif R. Bagaskara; et al. (2024). ( Molecular Rapid Test ( GeneXpert ) Result In Suspected Tuberculosis Patients At Buleleng General Hospital ). 8(2), 149– 154.

Alsayed, S. S. R., & Gunosewoyo, H. (2023). Tuberculosis: Pathogenesis, Current Treatment Regimens and New Drug Targets. International Journal of Molecular Sciences, 24(6). https://doi.org/10.3390/ijms24065202

Anam, K., & Rahmawati, E. (2022). Pemeriksaan Mikroskopis BTA Menggunakan Metode Pewarnaan Ziehl- Neeslen di RSUD Abdul Wahab Sjahranie Samarinda. Jurnal Teknologi Laboratorium Medik Borneo, 2(1), 54–61.

Bilgin, K., Yanik, K., Karadağ, A., Odabaşi, H., Taş, H., & Günaydin, M. (2016). Comparison of a real-time polymerase chain reaction-based system and Erlich–Ziehl–Neelsen method with culture in the identification of Mycobacterium tuberculosis. Turkish Journal of Medical Sciences, 46(1), 203–206.

Budiharjo, T., & Purjanto, K. A. (2016). Pengaruh Penanganan Sputum Terhadap Kualitas Sputum Penderita TBC Secara Mikroskpis Bakteri Tahan Asam. Jurnal Riset Kesehatan, 5(1), 40–44.

Dwipayana, I. M. G. (2022). Mengenali Gambaran Penyakit Tuberkulosis Paru Dan Cara Penanganannya. Widya Kesehatan, 4(1), 1–14.

Indra, S., Lestari, R., & Rinaldi, R. (2020). Hubungan Karakteristik Pasien Dengan Kejadian Tuberkulosis Di Rumah Sakit Tugu Jaya. Jurnal Kesehatan Medika Saintika, 11(2), 253–257.

Jannah, M., Wahyudi, A., Suryani, L., & Anggreny, D. E. (2024). Analisis Faktor Risiko Tuberkulosis Paru di Wilayah Kerja UPTD Puskesmas Lubuk Batang Kabupaten Ogan Komering Ulu Tahun 2024 Analysis of Pulmonary Tuberculosis Risk Factors in UPTD Lubuk Batang Community Health Center , Ogan Komering Ulu in 2024. Balaba, 20(1), 39–49.

Kassa, G. M., Merid, M. W., Muluneh, A. G., & Fentie, D. T. (2021). Sputum smear grading and associated factors among bacteriologically confirmed

pulmonary drug-resistant tuberculosis patients in Ethiopia. BMC Infectious Diseases, 21(1), 1–7.

Kementerian Kesehatan. (2022). Petunjuk Teknis Pemeriksaan Mikroskopis Tuberkulosis 2022. bit.ly/KIETBINDONESIA.

Kementerian Kesehatan Republik Indonesia. (2023). Petunjuk Teknis Pemeriksaan Tuberkulosis Menggunakan Tes Cepat Molekuler GeneXpert. 241.

Lailatul, I., & Ziyadatur, M. (2024). Literature review : faktor risiko lingkungan kejadian tuberkulosis. 4(2), 476–483.

Lestari, H. (2024). Epidemiological Analysis Of Tuberculosis Incidents In Southwest Sulawesi Province In 2021-2023. Variable Research Journal, 01(02), 802–810.

Lestari, L., Sijid, S. A., & Hidayat, K. S. (2023). Hasil diagnostik Mycobacterium tuberculosis dari sputum penderita batuk ≥ 2 minggu dengan pewarnaan Ziehl-Neelsen di Balai Besar Kesehatan Paru Masyarakat Makassar. Filogeni: Jurnal Mahasiswa Biologi, 3(1), 12–17.

Luies, L., & Preez, I. du. (2020). The echo of pulmonary tuberculosis: Mechanisms of clinical symptoms and other disease-induced systemic complications. Clinical Microbiology Reviews, 33(4), 1–19.

M. Sabir, & Sarifuddin. (2023). Analisis Faktor Risiko Tingginya kasus Tuberkulosis Paru di Indonesia : Literatur Revieu. Jurnal Kolaboratif Sains, 6(6), 453–468.

Mahendrani, C. R. M., Subkhan, M., Nurida, A., Prahasanti, K., & Levani, Y. (2020). Analisis Faktor Yang Berpengaruh Terhadap Konversi Sputum Basil Tahan Asam Pada Penderita Tuberkulosis. Al-Iqra Medical Journal : Jurnal Berkala Ilmiah Kedokteran, 1(2), 1–9.

Malewa, F., & Yartin, S. (2024). Asuhan Keperawatan Keluarga pada An . Z yang Menderita Penyakit Tuberkulosis ( TB ) Family Nursing Care in An . Z Suffering From Tuberculosis ( TB ) Jurnal Kolaboratif Sains ( JKS ). 7(3), 1274–1284.

Nuryaningsih, E., Mulyanto, A., Kurniawan, & Mujahid, I. (2023). Proporsi hasil pemeriksaan pewarnaan ziehl neelsen dengan tes cepat molekuler pada tuberkulosis paru. Journal of Medical Laboratory and Science, 3(2), 3–8.

Perhimpunan Dokter Paru Indonesia. (2021). Tuberkulosis Pedoman Diagnosis dan Penatalaksanaan di Indonesia. In Perhimpunan Dokter Paru Indonesia (Vol. 001, Issue 2014).

PNPK. (2015). Pedoman Nasional Pelayanan Kedokteran Tata Laksana Tuberkulosis.

Qurrohman, M. T. (2024). Uji Kesuaian Pemeriksaan BTA Metode Ziehl Neelsen dengan GeneXpert pada Pasien Tuberkulosis Paru. 465–470.

Ramadhan, R., Fitria, E., & Rosdiana. (2017). Deteksi Mycobacterium tuberculosis dengan pemeriksaan paru di puskesmas darul imarah. SEL Jurnal Penelitian Kesehatan, 4(2), 74–81.

Ratna, Fitriana, V., Khamdannah, E. N., & Fitriana, A. A. (2023). Pencegahan Penularan TBC Melalui Implementasi Cekoran Bu Titik (Cegah Resiko Penularan Melalui Batuk Efektif dan Etika Batuk) pada Remaja di SMAN2 Kudus. Jurnal Pengabdian Kesehatan, 6(1), 77–87.

Revi, C., Mahendrani, M., Subkhan, M., Nurida, A., Prahasanti, K., Levani, Y., & Surabaya, U. M. (2020). Analisis faktor yang berpengaruh terhadap konversi sputum basil tahan asam pada penderita tuberkulosis. 3(1), 1–9.

Rsudprovsumsel. (2021) http://rsud.sumselprov.go.id/profile/detail/selayang- pandang-tentang-rsud-siti-fatimah-provinsi-sumatera-selatan

Rsudprovsumsel.2025.http://rsud.sumselprov.go.id/media/detil/visi-dan-misi- rsud-siti-fatimah-provinsi-sumatera-selatan

Russell, D. G., Cardona, P. J., Kim, M. J., Allain, S., & Altare, F. (2017). Foamy macrophages and the progression of the human tuberculosis granuloma. Nature Immunology, 10(9), 943–948.

Sari, G. K., Sarifuddin, & Setyawati, T. (2022). Tuberkulosis Paru Post Wodec Pleural Efusion: Laporan Kasus Pulmonary Tuberculosis Post Wodec Pleural Effusion: Case Report. Jurnal Medical Profession, 4(2), 174–182.

Siregar, T., & Aryayuni, C. (2019). Pengaruh Fisioterapi Dada Terhadap Pengeluaran Sputum Pada Anak Dengan Penyakit Gangguan Pernafasaan Di Poli Anak RSUD Kota Depok. Jurnal Keperawatan Widya Gantari Indonesia, 2(2), 34–42.

Syafwan, M. K. R., Miswarti, & Afnuhazi, R. (2022). Faktor-faktor yang Mempengaruhi Hasil Belajar Kelas VI SDN 20 Indarung Padang. Jurnal Kesehatan Saintika Meditory, 4(4657), 78–84.

Tamunu, M. sarra, Pareta, D. N., Hariyadi, H., & Karauwan, F. A. (2022). Skrining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Daun Benalu Pada Kersen Dendrophtoe pentandra (L.) Dengan Metode 2,2- diphenyl -1- Picrylhydrazyl (DPPH). Biofarmasetikal Tropis, 5(1), 79–82.

Utami, P. R., Amelia, N., Susanto, V., & Adfar, T. D. (2021). Pemeriksaan GeneXpert Terhadap Tingkat Positivitas Pemeriksaan Basil Tahan Asam (BTA) Metode Ziehl Neelsen Pada Penderita Suspek TB Paru. JURNAL KESEHATAN PERINTIS (Perintis’s Health Journal), 8(1), 82–90.

Zaporojan, C., Csep, A., Zaporojan, N., Negrean, R. A., Hodis, R., & Zaha, D. C. (2024). Evolution of Laboratory Diagnosis of Tuberculosis. 388–416.